Mass Spectrometry-based Methods of Proteome Analysis
نویسندگان
چکیده
1 Principles and Instrumentation 3 1.1 Proteome and Proteomics 3 1.2 Need for Large-scale Analyses of Gene Products at the Protein Level 3 1.3 General Problems in Proteome Analyses 4 1.4 MS Instrumentation 5 1.4.1 Ionization Methods 6 1.4.2 Mass Analyzers 8 1.4.3 MS/MS 9 1.5 Methods of Sample Fractionation 10 1.5.1 2D-electrophoretic Separation of Complex Protein Mixtures 11 1.5.2 Liquid-phase Separation Methods 12 1.5.3 Affinity Methods (Epitope Tagging) 13 1.6 Protein Identification by MS 14
منابع مشابه
Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry
Until recently, Cryptosporidium was thought to be a single species genus. Molecular studies now showthat there are at least 10 valid species of this parasite. Among them, two morphologically identical species, C.hominis and C. parvum are the most pathogenic identified to date and share 97% of identical genomes.Post-genomic analyses is therefore necessary to explore further the...
متن کاملProteome Profiling by Multidimensional Protein Separation and Automated Lab-on-a-Chip Technology
Technologies and methodologies for proteomic studies often involve a combination of protein fractionation, identification, and quantitation by liquid chromatography tandem mass spectrometry (LC/MS/MS) and in many cases pattern comparison of mass spectra from a complex sample. A common goal of these efforts is the discovery and validation of biomarkers. While current mass spectrometry-based prot...
متن کاملProtein identification and expression analysis using mass spectrometry.
The identification and quantification of the proteins that a whole organism expresses under certain conditions is a main focus of high-throughput proteomics. Advanced proteomics approaches generate new biologically relevant data and potent hypotheses. A practical report of what proteome studies can and cannot accomplish in common laboratory settings is presented here. The review discusses the m...
متن کاملStandardized approach to proteome profiling of human serum based on magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
BACKGROUND Magnetic bead purification for the analysis of low-abundance proteins in body fluids facilitates the identification of potential new biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The aims of our study were to establish a proteome fractionation technique and to validate a standardized blood sampling, processing, and storage ...
متن کاملProteome Analysis of Rat Hippocampus Following Morphine-induced Amnesia and State-dependent Learning
Morphine’s effects on learning and memory processes are well known to depend on synaptic plasticity in the hippocampus. Whereas the role of the hippocampus in morphine-induced amnesia and state-dependent learning is established, the biochemical and molecular mechanisms underlying these processes are poorly understood. The present study intended to investigate whether administration of morphine ...
متن کامل